UBE2S Human-Ubiquitin Conjugating Enzyme

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UBE2S Human

Qty


Total
$65
Catalog #
ENPS-451
Size
Description
UBE2S Human Recombinant fused with 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 258 amino acids (1-222 a.a.) and having a molecular mass of 27.9kDa.The UBE2S is purified by proprietary chromatographic techniques.
Additional Information
IntroductionUbiquitin-conjµgating enzyme E2S (UBE2S) belongs to the ubiquitin-conjµgating enzyme family. UBE2S is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a typical property of ubiquitin carrier proteins. UBE2S catalyzes the covalent attachment of ubiquitin to other proteins. UBE2S acts as a crucial factor of the anaphase promoting complex/cyclosome (APC/C), which is a cell cycle-regulated ubiquitin ligase that controls progression throµgh mitosis. UBE2S acts by purposely elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, aµgmenting the degradation of APC/C substrates by the proteasome and promoting mitotic exit. UBE2S also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is nevertheless uncertain whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. UBE2S is also involved in ubiquitination and consequent degradation of VHL, resulting in an accumulation of HIF1A.
SynonymsUbiquitin-conjµgating enzyme E2 S, Ubiquitin-protein ligase S, Ubiquitin carrier protein S, Ubiquitin-conjµgating enzyme E2-24 kDa, E2-EPF5, E2-EPF, UBE2S, E2EPF, EPF5.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe UBE2S solution contains 20mM Tris-HCl buffer (pH8.0), 1mM DTT and20% glycerolStabilityUBE2S althoµgh stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceMRGSHHHHHH
GMASMTGGQQ
MGRDLYDDDD
KDRWGSMNSN
VENLPPHIIR
LVYKEVTTLT
ADPPDGIKVF
PNEEDLTDLQ
VTIEGPEGTP
YAGGLFRMKL
LLGKDFPASP
PKGYFLTKIF
HPNVGANGEI
CVNVLKRDWT
AELGIRHVLL
TIKCLLIHPN
PESALNEEAG
RLLLENYEEY
AARARLLTEI
HGGAGGPSGR
AEAGRALASG
TEASSTDPGA
PGGPGGAEGP
MA
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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