β-actin-Monoclonal Antibodies

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β-actin

Qty


Total
$135
Catalog #
AC004
Antibody Type
Monoclonal Antibody
Gene ID
60
Swiss Prot
P60709
Size
Species
Mouse
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:5000 - 1:10000 IHC 1:50- 1:100 IF 1:50- 1:200
Calculated MW42kDa
Observed MWRefer to Figures
ImmunogenFusion protein of human β-actin
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationh
SynonymACTB;PS1TP5BP1 ;beta-actin; β-actin
Images
  • AC004: image 1

    Western blot analysis of extracts of various cells, using β-actin antibody at dilution of 1:10000.

Background

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha ( ACTC1, cardiac muscle 1), alpha 1 ( ACTA1, skeletal muscle) and 2 ( ACTA2, aortic smooth muscle), beta ( ACTB), gamma 1 ( ACTG1) and 2 ( ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins.

Protocol

N/A

MSDS
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