Monoclonal Antibody Production | Monoclonal Antibody

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Quotations & Ordering:

For quotations, please use our online quotation form, and you may also contact us by

[email protected]

Phone:

+1888-754-5670(Toll-free)
+1857.259.4898 (Int’l)

Fax:

+1888-754-5670,

Our customer service representatives are available 24 hours, Monday through Friday to assist you.
NeoScientific is a leader in custom monoclonal antibody development. Our antibody team strives to deliver successful projects to customers in the most cost-effective way. Our monoclonal antibody service delivers on the most difficult projects. We have created antibodies for numerous applications including functional assays, immuneprecipitation, IHC, IF, flow cytometry, Western blot, and ELISA. You can review more information we have on monoclonal antibodies in our blog.
Guarantees:

We guarantee positive clones for monoclonal antibodies development if antigens were prepared.

Services Include:

NeoScientific offers a variety of hybridoma development packages and monoclonal antibody production services. Our scientists will help you select the best service for you

NeoScientific offers a variety of hybridoma development packages and monoclonal antibody production services. Our scientists will help you select the best service for you.
1. Basic MonoclonalAntibody Package (Mouse/Rat)
Package includes:

Immunization and ELISA screening
Fusion and screening
Subcloning, expansion, cryopreservation

You will receive (in 4-6 months):

Up to 5 positive hybridoma clones
Two vials of cell culture for each clone
5 ml of culture supernatant

2. Complete PeptideMonoclonal Antibody Package (Mouse/Rat)
Package includes:

Peptide synthesis
Immunization and ELISA screening
Fusion and screening
Subcloning, expansion, cryopreservation

You will receive (in 5-7 months):

Up to 5 positive hybridoma clones
Two vials of cell culture for each clone
5 ml of culture supernatant
5 mg of unconjugated peptide

3. Phospho-SpecificMonoclonal Antibody Package (Mouse/Rat)
Package includes:

Peptide synthesis
Immunization and ELISA screening
Fusion and screening
Subcloning, expansion, cryopreservation

You will receive (in 5-7 months):

Up to 5 positive hybridoma clones
Two vials of cell culture for each clone
5 ml of culture supernatant
5 mg of phospho-peptide
5 mg of unmodified peptide

4. Anti-idiotypeMonoclonal Antibody Package (Mouse/Rat)
Package includes:

Immunogen preparation
Immunization and ELISA screening
Fusion and screening
Subcloning, expansion, cryopreservation

You will receive (in 5-7 months):

Up to 5 positive hybridoma clones
Two vials of cell culture for each clone
5 ml of culture supernatant

Note:

1. We provide free cell line storage service for 6 months from the date of cell freezing. If customers require continued storage service after the end of six-month period, the charges are set at $60 per cell line per year.

2. If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products.

NeoScientific's standard option for monoclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.

The protocol uses animals that are Specific Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA).

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (5mL per rabbit)
Primary Injection Day 1 Immunize with 0.50mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Bleed (~25mL per rabbit)
3rd Booster Day 42 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56, 58 Two bleeds (~50mL total per rabbit)
ELISA Test Day 60 ELISA titration (results available online);
Verify disposition of rabbits; decide to continue or terminate
Purification Optional 6-10 mg purified antibodies
Quote
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 245 First Street, 18th Floor, Cambridge, MA 02142 USA.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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