Yeast Expression System | Protein Services

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Quotations & Ordering:

For quotations, please use our online quotation form, and you may also contact us by

[email protected]

Phone:

+1888-754-5670(Toll-free)
+1857.259.4898 (Int’l)

Fax:

+1888-754-5670,

Our customer service representatives are available 24 hours, Monday through Friday to assist you.
Yeast System

The yeast expression system is the most economical eukaryotic expression system for both secreted and intracellular proteins. It is ideally suited for large-scale production of recombinant eukaryotic proteins.Our yeast expression system provides the advantages of both prokaryotic and eukaryotic expression systems: high expression level, low cost, ease of scale up, and proper protein folding. We have multiple strains and expression vectors to help identify the highest yielding conditions. We also utilize robust secretion signal sequences to ensure the highest secretion of recombinant protein. Our customers can also choose constitutive or inducible expression systems.

Phase I: Gene Synthesis and Subcloning

We can take a customer provided cDNA or optimize and synthesize any desired coding sequence and subclone it into a yeast expression vector. Once cloned, all constructs are restriction digest and sequence verified.

Phase II: Pilot Protein Expression and Purification

The appropriate yeast strain is transformed with the linearized expression construct and positively selected transformants screened for integration by PCR. Small scale induction experiments are conducted to identify high-expressing transformants and optimize expression and purification conditions.

Phase III: Protein Expression and Purification

We use our optimized conditions to scale up expression to one liter cultures. We access multiple purification strategies to isolate several milligrams of product at this stage. If larger quantities are required, we can scale up to 35-500L culture sizes.

Purification Capabilities
Affinity Chromatography
Ni-NTA, Talon resin, Glutathione Sepharose, Heparin Sepharose, Streptavidin Sepharose, anti-Flag, Protein A, Protein G
Ion Exchange Chromatography
Mono-Q, Q Sepharose Fast Flow, Resource Q, Q Sepharose XL, ANX, DEAE Sepharose, SP Sepharose, CM Sepharose
Hydrophobic Interaction Chromatography
Phenyl and (CH2)n Sepharose
Size Exclusion Chromatography
Additional Services
Tag removal
Endotoxin Removal
Protein solublization and refolding
Metabolic labeling: Stable isotope labeling with 13C and 15N.
Protein Service Quotation Request Form
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 245 First Street, 18th Floor, Cambridge, MA 02142 USA.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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