Polyclonal Antibody Prodcution | Polyclonal Antibody

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Quotations & Ordering:

For quotations, please use our online quotation form, and you may also contact us by

[email protected]

Phone:

+1888-754-5670(Toll-free)
+1857.259.4898 (Int’l)

Fax:

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Our customer service representatives are available 24 hours, Monday through Friday to assist you.
Polyclonal antibodies recognize multiple epitopes on a single immunogen and are composed of several immunoglobulin subtypes. They can be used in multiple applications including Western blots, ELISA, and IHC. We guarantee that your polyclonal antibody produced in any host will have an ELISA titer of 1:32,000 or better if peptide antigens were designed, synthesized and conjugated by NeoBioLab. For any protein antigen (3 mg, >80% purity, 90% preferred) we guarantee a polyclonal antibody with positive Western blot results and an ELISA titer of 1:32,000 or better for any host. We will restart the project free of charge until our guarantee is met. We have more informational regarding polyconal antibodies in our blog.
NeoScientific offers a variety of polyclonal antibody production packages. Our experienced sales account managers will guide you toward the best selection for your specific needs.
Express Polyclonal Antibody Service (Cat.No.: PA001, Duration: 7-10 weeks)
Service includes:

Protein/Peptide Antigen (Additional service if client does not provide antigen)
Conjugation to Carrier
Immunization of 2 rabbits
ELISA test
(If the antigen is provided by the customer: 5 mg of peptide or 5 mg of protein, purity >90% is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents)

Package includes:

60 ml antisera
ELISA results

Basic Polyclonal Antibody Service (Cat.No.: PA002, Duration: 14-16 weeks)
Service includes:

Protein/Peptide Antigen (Additional service if client does not provide antigen)
Conjugation to Carrier
Immunization of 2 rabbits
ELISA test
(If the antigen is provided by the customer: 5 mg of peptide or 5 mg of protein, purity >90% is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents)

Package includes:

70-100 ml antisera
ELISA results

Standard Polyclonal Antibody Service (Cat.No.: PA003, Duration: 15-17 weeks)
Service includes:

Peptide synthesis and carrier conjugation
Immunization of two rabbits
4 immune bleeds
Final bleed
Peptide affinity purification
ELISA test

Package includes:

3-5 mg affinity-purified antibodies (>1:20,000)
5 mg of unconjugated peptide
1 ml of pre-immune serum
ELISA, MS and HPLC results

Additional services:
4-Week extension:
1 additional immunization and 2 blood collections for ~80-100ml of antisera
Affinity purification:
Affinity purification of 25ml of sera. Includes the affinity column.
ELISA:
On any bleed or purified antibody according to customer request.
Western blot:
Assay of antisera or purified antibodies according to customer request
Immunohistochemistry:
Assay of purified antibodies against tissue sections according to customer request
Peptide Synthesis:
Full range of custom peptide services available
Protein Antigens:
Full range of custom protein services available

NeoScientific's standard option for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.

The protocol uses two New Zealand White rabbits that are Specific Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA).

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (5mL per rabbit)
Primary Injection Day 1 Immunize with 0.50mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Bleed (~25mL per rabbit)
3rd Booster Day 42 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56, 58 Two bleeds (~50mL total per rabbit)
ELISA Test Day 60 ELISA titration (results available online);
Verify disposition of rabbits; decide to continue or terminate
Purification Optional 6-10 mg purified antibodies
Quote
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 245 First Street, 18th Floor, Cambridge, MA 02142 USA.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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