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Our optimized custom lentiviral vector production and strict quality control systems provide customers a high titer of functional recombinant lentiviral vectors. Viral titers are determined by two methods: functional (infectious) titer (TU/mL) and physical titer (VP/mL). Physical titer is calculated by the level of protein, such as p24, or viral nucleic acid. The functional titer, a calculation of the active virus that can infect cells, is much less than the physical titer (100-1000 fold lower). Direct functional titer is an accurate solution for testing the MOI but is time consuming and not feasible. The physical titer is sufficient for most lentiviral experiments. Our main titration procedure is to determine the quantity of physical lentiviral particles. We can also calculate the functional lentiviral vectors with additional cost if required by customers.
|Service Package||Vector Titer*||Volume||Turnaround||Price*|
|Crude||N/A||10 ml||2-3 w||$450|
|Low-Titer||>107||1 ml||2-3 w||$1290|
|Medium-Titer||>108||200 µl||2-3 w||$1590|
|High-Titer||>109||200 µl||2-3 w||$2290|
|Ultra-High-Titer||>1010||20 µl||2-3 w||$2490|
|Custom-Titer & Scale||Custom||Custom||2-3 w||Quote|
We will subclone the gene of interest or shRNA into the lentiviral vector, prepare high quality plasmid DNA, transfect the packaging cell line, and harvest the lentivirus particle, followed by determining the concentration and titration. You will receive lentiviral vectors after titration.
We will prepare endotoxin-free plasmid, transfect the packaging cell line for lentivirus production, and harvest the lentivirus particle, followed by determining the concentration and titration. You will receive lentiviral vectors after titration.