Lentivirus Packaging Service | Lentivirus Manufacturing

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Quotations & Ordering:

For quotations, please use our online quotation form, and you may also contact us by

[email protected]

Phone:

+1888-754-5670(Toll-free)
+1857.259.4898 (Int’l)

Fax:

+1888-754-5670,

Our customer service representatives are available 24 hours, Monday through Friday to assist you.

Lentivirus Manufacturing

Lentiviral vector is an efficient solution for delivering genes into mammalian cells. Neo’s Lentiviral Packaging Systems utilize the newest generation lentiviral technology to create recombinant lentivirus. We provide flexible and high quality lentiviral packaging services, from construct design to lentiviral vector production, concentration, and titration.

Quality Control Systems

Our optimized custom lentiviral vector production and strict quality control systems provide customers a high titer of functional recombinant lentiviral vectors. Viral titers are determined by two methods: functional (infectious) titer (TU/mL) and physical titer (VP/mL). Physical titer is calculated by the level of protein, such as p24, or viral nucleic acid. The functional titer, a calculation of the active virus that can infect cells, is much less than the physical titer (100-1000 fold lower). Direct functional titer is an accurate solution for testing the MOI but is time consuming and not feasible. The physical titer is sufficient for most lentiviral experiments. Our main titration procedure is to determine the quantity of physical lentiviral particles. We can also calculate the functional lentiviral vectors with additional cost if required by customers.


Price System
Service Package Vector Titer* Volume Turnaround Price*
Crude N/A 10 ml 2-3 w $450
Low-Titer >107 1 ml 2-3 w $1290
Medium-Titer >108 200 µl 2-3 w $1590
High-Titer >109 200 µl 2-3 w $2290
Ultra-High-Titer >1010 20 µl 2-3 w $2490
Custom-Titer & Scale Custom Custom 2-3 w Quote
Note:
  1. Customer should provide enough amount of sterile, endotoxin-free ready-for-transfection transfer vector DNA for virus production. We can provide custom vector construction and gene synthesis services at additional cost.
  2. The p24 ELISA kit is the most commonly published method for measuring lentiviral titer. We will follow this method to calculate the titer.
Disclaimer: For sizes > 9 kb, packaging efficiency can be compromised. Also, our guarantee may not apply if the vector contains sequences with very high GC content (> 70% across a few hundred bases) that can reduce packaging efficiency, or genes toxic to packaging cells.

Virus Packaging Process

If you need us to build lentiviral vector for your gene, please:
  1. Provide a target gene sequence and the lentiviral vector information. We will synthesize the gene and subclone it for you.
  2. If you have a target gene, please send us 1~5 µg of plasmid or bacterial culture for your gene.

We will subclone the gene of interest or shRNA into the lentiviral vector, prepare high quality plasmid DNA, transfect the packaging cell line, and harvest the lentivirus particle, followed by determining the concentration and titration. You will receive lentiviral vectors after titration.


If the lentiviral construct is ready, please provide us:
  1. The construct map and sequence.
  2. 1~5 µg of your lentiviral construct plasmid DNA or bacterial culture.

We will prepare endotoxin-free plasmid, transfect the packaging cell line for lentivirus production, and harvest the lentivirus particle, followed by determining the concentration and titration. You will receive lentiviral vectors after titration.

  • Day1

    • Transfect 293F cells with combinations of packaging and lenti vectors

  • Day4

    • Harvest conditioned medium

  • Day5

    • TPurify or precipitate virus particles
    • Determine virus titers

Features

  1. Chem-Lenti™ (in-house chemically defined media) is used to grow and transfect 293F cells. No animal component in crude virus harvest.
  2. FusureTrans™ (innovative reagent for lentiviral packaging) for the transduction processes.
  3. Consistent infectious units exceeding 1e7/ml virus in conditioned medium. We can further concentrate and purify the lentivirus.
  4. Provide proprietary reagent to pull down viruses during the transduction process.
  5. Employ in-house lentiviral vector for highly efficient packaging.

Innovations

Our team is dedicated to lentiviral vector development and virus packaging for genetic engineering. We now provide our superior lentiviral technologies for public use. Our PhD scientists have been dedicated to virus pseudotyping research for more than 15 years and developed unique technologies optimizing packaging efficiency, including in-house vectors, Chem-Lenti™, and FUSURE Trans™ Technologies.
Vector
We provide services from gene synthesis to lentivirus packaging services. Please note, use of our M1 Lenti Viral Vector will ensure consistent packaging results. Because packaging efficiency depends on the gene insert and type of lentiviral vector, we may not be able to assure consistent packaging using customer’s vector. For 1e7 titer range, the insert shall be less than 1.5 kb.
Chem-Lenti™ chemically defined media
Our in-house chemically defined media Chem-Lenti™ generates high titers of lentivirus.
Titers of the packaged virus are determined by serial dilutions and subsequent infection of 293F cells.
Titers are estimated by averaging cells infected when the infection ratios are in the 5-20% range. (See formula below for the 1:81 fold dilution)
Titer = 200,000 cells x 13.25% x 81 / 0.2 ml (virus dilution used) = 10.7 M/ml

Neg control

Undiluted

1:9 diluted

1:81 diluted


NEO’s lenti expression system provides rapid stable


We Produce Consistent Results


FuSure Reagent Pulls Down virus and Facilitates Transduction
Infection achieved by regular incubation for attached cells, or by 30 – 60 min spin at 300 g for suspension cells. No high speed spin is needed.

Price Quotation Form
To receive a quote, please fill in as much information about your project as possible. Not all of the information is required, but by providing more information you will help us to route your inquiry to the appropriate person.
Upload attachment file format xls, txt, scv, each file maximum 1M

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