Mammalian Cell Expression | Protein Services

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Quotations & Ordering:

For quotations, please use our online quotation form, and you may also contact us by

[email protected]

Phone:

+1888-754-5670(Toll-free)
+1857.259.4898 (Int’l)

Fax:

+1888-754-5670,

Our customer service representatives are available 24 hours, Monday through Friday to assist you.
Mammalian System

Mammalian cells are the preferred expression platform for proteins requiring fully mammalian post-translational modifications. As such, they are commonly used for the production of antibodies and therapeutic proteins. They are also preferred when the downstream application for a recombinant protein is in a functional mammalian cell-based assay. At NeoBioLab we produce proteins in both transient and stable formats using HEK293 or CHO cells. We utilize proprietary expression vectors to drive high levels of expression and regularly return >10mg/L of recombinant protein. Our superior capacity allows us to conduct tens of transient expression projects simultaneously and our serum-free suspension culture format allows for large-scale production yielding grams of the desired protein.

Phase I: Gene Synthesis and Subcloning

We can take a customer provided cDNA or optimize and synthesize any desired coding sequence and subclone it into a mammalian expression vector. Once cloned, all constructs are restriction digest and sequence verified.

Phase II: Pilot Protein Expression and Purification

Generally, we conduct small scale expression and purification trials to identify the best clones and/or expression conditions along with optimal protein isolation and purification procedures. We use these conditions to produce approximately 100 micrograms of protein.

Phase III: Protein Expression and Purification

For larger protein requests, we scale up expression using optimized conditions. We utilize 20L Wave Bag systems to produce gram quantities of the desired protein.

Purification Capabilities
Affinity Chromatography
Ni-NTA, Talon resin, Glutathione Sepharose, Heparin Sepharose, Streptavidin Sepharose, anti-Flag, Protein A, Protein G
Ion Exchange Chromatography
Mono-Q, Q Sepharose Fast Flow, Resource Q, Q Sepharose XL, ANX, DEAE Sepharose, SP Sepharose, CM Sepharose
Hydrophobic Interaction Chromatography
Phenyl and (CH2)n Sepharose
Size Exclusion Chromatography
Additional Services
Tag removal
Endotoxin Removal
Protein solublization and refolding
Metabolic labeling: Stable isotope labeling with 13C and 15N.
Protein Service Quotation Request Form
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 245 First Street, 18th Floor, Cambridge, MA 02142 USA.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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