|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF IP|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50|
|Observed MW||Refer to Figures|
|Immunogen||A synthetic peptide of human AFP|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||AFP ; FETA; Alpha fetoglobulin; alpha fetoprotein; Alpha 1 fetoprotein; HPAFP|
Western blot analysis of extracts of various cells, using AFP antibody.
Western blot analysis of extracts of A549 cells, using AFP antibody.
Immunofluorescence analysis of HeLa cell using AFP antibody. Blue: DAPI for nuclear staining.
Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the serum of the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Elevated serum levels are predictive of hepatocellular carcinoma (3).
Neo Scientific welcomes feedback from its customers.
If you have used an our product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information.
If you have any additional inquiries please email technical services at firstname.lastname@example.org
Thank you for your support.