APC-Polyclonal Antibodies

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APC

Qty


Total
$380
Catalog #
A0021
Antibody Type
Polyclonal Antibody
Gene ID
324
Swiss Prot
P25054
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Rat
Tested applicationsWB IHC ICC IP
Recommended DilutionWB 1:500 - 1:1000 IHC 1:50 - 1:100 ICC 1:50 - 1:100 IP 1:25 - 1:50
Calculated MW312/160kDa
Observed MWRefer to Figures
ImmunogenA synthetic peptide of human APC
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SynonymAPC ; Deleted in polyposis 2.5; DP3; DP2; GS; DP2.5
Images
  • A0021: image 1

    Western blot analysis of extracts of 293 cell line using APC antibody.

Background

The Adenomatous Polyposis Coli (APC) tumor suppressor gene is mutated in most familial and sporadic colorectal cancers and encodes a large cytoplasmic protein that is implicated in cell migration, cell adhesion, and proliferation (1). APC binds directly to microtubules and lack of APC leads to defective mitotic spindles and aneuploidy due to missegregation of chromosomes (2). APC is well characterized as a scaffolding protein, binds to β-catenin, and is involved in the regulation of its intracellular concentration. In the absence of a Wnt signal, GSK-3β phosphorylates all three members of the APC-β-catenin-axin complex and this phosphorylation of β-catenin creates a recognition site for ubiquitin, the signal for proteasome-mediated degradation. In the presence of a Wnt signal, dishevelled inactivates GSK-3β and β-catenin coordinates gene transcription of proteins important for the control of cell cycle progression and proliferation, such as cyclin D1 and c-Myc (3).

Protocol

N/A

MSDS
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