|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:10 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human APP|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||APP;AAA;ABETA;ABPP;AD1;APPI;CTFgamma;CVAP;PN2;amyloidP; Amyloid beta A4 protein; A4; Beta Amyloid|
Western blot analysis of extracts of various cell lines, using APP antibody.
Immunohistochemistry of paraffin-embedded mouse spleen using APP antibody.
Immunohistochemistry of paraffin-embedded human stomach using APP antibody.
Immunohistochemistry of paraffin-embedded rat brain using APP antibody.
Immunohistochemistry of paraffin-embedded rat spleen using APP antibody.
Immunohistochemistry of paraffin-embedded human esophageal cancer using APP antibody.
Immunohistochemistry of paraffin-embedded human esophagus using APP antibody.
Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (Aβ), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle-dependent and peaks during G2/M-phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).
Neo Scientific welcomes feedback from its customers.
If you have used an our product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information.
If you have any additional inquiries please email technical services at firstname.lastname@example.org
Thank you for your support.