ARFGAP1-Polyclonal Antibodies

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ARFGAP1

Qty


Total
$220
Catalog #
A7118
Antibody Type
Polyclonal Antibody
Gene ID
55738
Swiss Prot
Q8N6T3
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Calculated MW45kDa
Observed MWRefer to figures
ImmunogenRecombinant protein of human ARFGAP1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SynonymARF1GAP; HRIHFB2281;
Images
  • A7118: image 1

    Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody.

  • A7118: image 2

    Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody at dilution of 1:100 (x40 lens).

  • A7118: image 3

    Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody at dilution of 1:100 (x40 lens).

  • A7118: image 4

    Immunofluorescence analysis of A549 cell using ARFGAP1 antibody. Blue: DAPI for nuclear staining.

Background

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.

Protocol

N/A

MSDS
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