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Reactivity | Human Mouse Rat |
Tested applications | WB IHC ICC IF |
Recommended Dilution | WB 1:500 - 1:1000 IHC 1:50 - 1:200 ICC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 14 kDa |
Observed MW | Refer to figures |
Immunogen | A specific peptide of Acetyl-Histone H2A-K9 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | H2A.1; H2A.2; H2A/a; H2AFA; |
Immunohistochemistry of paraffin-embedded mouse colon using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse thyroid using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunofluorescence
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
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