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Reactivity | Human Mouse Rat Other (Wide Range) |
Tested applications | WB IHC IF IP ChIP ChIPseq |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 CHIPseq 1:20 - 1:50 |
Calculated MW | 16kDa |
Observed MW | Refer to figures |
Immunogen | A synthetic peptide of human Acetyl-Histone H3-K9 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | q |
Synonym | H3K9ac; H3t; H3.4; H3/g; H3FT; H3K9ac |
Western blot analysis of extracts of various cell lines and H3 protein expressed in E.coli., using H3K9ac antibody.
Immunofluorescence analysis of HeLa cell using H3K9ac antibody. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis extracts of 293 cell line, using H3K9ac antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
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