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Reactivity | Human Mouse Rat |
Tested applications | WB IHC ICC IP |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 ICC 1:50 - 1:200 IP 1:20 - 1:50 |
Calculated MW | 11 kDa |
Observed MW | Refer to figures |
Immunogen | A specific peptide of Acetyl-Histone H4-K5 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | H4/I; H4FI; |
Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H4-K5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Acetyl-Histone H4-K5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H4-K5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse colon using Acetyl-Histone H4-K5 antibody at dilution of 1:100 (40x lens).
Immunofluorescence
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
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