|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:100|
|Observed MW||Refer to figures|
|Immunogen||Recombinant protein of human BCKDK|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Western blot analysis of extract of various cells, using BCKDK antibody.
Immunohistochemistry of paraffin-embedded human liver injury using BCKDK antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat lung using BCKDK antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat kidney using BCKDK antibody at dilution of 1:100 (x40 lens).
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
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