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Reactivity | Human Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
Calculated MW | 22kDa |
Observed MW | Refer to Figures |
Immunogen | A synthetic peptide of human BCL2 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | bi |
Synonym | Bcl-2;PPP1R50; |
Western blot analysis of extracts of various cell lines, using Bcl-2 antibody.
Immunohistochemistry of paraffin-embedded human colon using BCL2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using BCL2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using BCL2 antibody.
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
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