BRAF-Polyclonal Antibodies

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BRAF

Qty


Total
$220
Catalog #
A0038
Antibody Type
Polyclonal Antibody
Gene ID
673
Swiss Prot
P15056
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman
Tested applicationsWB IHC
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200
Calculated MW94kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human BRAF
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationboq
SynonymB-RAF1;BRAF1;NS7;RAFB1;Proto-oncogeneB-Raf;p94;v-Raf murine sarcoma viral oncogene homolog B1 antibody;
Images
  • A0038: image 1

    Western blot analysis of extracts of various cells, using BRAF antibody.

Background

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system. It has been observed that MAPK is activated by B-Raf in response to nerve growth factor. More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity. Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Protocol

N/A

MSDS
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