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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 63kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human CALD1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22 |
Western blot analysis of extracts of various cell lines, using CALD1 antibody.
Immunohistochemistry of paraffin-embedded human liver injury using CALD1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CALD1 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of A549 cell using CALD1 antibody. Blue: DAPI for nuclear staining.
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
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