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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 32kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human CASP3 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | bi |
Synonym | CASP3;CPP32;CPP32B;SCA-1;Caspase-3;APOPAIN; |
Western blot analysis of extracts of various cell lines using CASP3 antibody.
Western blot analysis of extracts of various cell lines using CASP3 antibody.
Immunohistochemistry of paraffin-embedded Rat lung using CASP3 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of U20S cell using CASP3 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cell using CASP3 antibody. Blue: DAPI for nuclear staining.
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).
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