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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
Calculated MW | 33kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human CCNC |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | CycC; |
Western blot analysis of extracts of various cell lines, using CCNC antibody.
Immunohistochemistry of paraffin-embedded rat brain using CCNC antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human lung cancer using CCNC antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using CCNC antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of HeLa cell using CCNC antibody. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene.
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