CD3E-Polyclonal Antibodies

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CD3E

Qty


Total
$220
Catalog #
A1753
Antibody Type
Polyclonal Antibody
Gene ID
916
Swiss Prot
P07766
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Calculated MW23kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human CD3E
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationb
SynonymCD3ε; FLJ18683; T3E; TCRE;
Images
  • A1753: image 1

    Western blot analysis of extracts of Jurkat cell lines, using CD3E antibody.

  • A1753: image 2

    Immunofluorescence analysis of U2OS cell using CD3E antibody. Blue: DAPI for nuclear staining.

Background

When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline-region is exposed and then associates with the adapter protein Nck (5).

Protocol

N/A

MSDS
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