|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:100 - 1:200|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant Protein of human CDCA8|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||CDCA8;BOR;BOREALIN;FLJ10468;FLJ12042 ; Borealin|
Western blot analysis of HEX293 cell lysate using CDCA8 antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using CDCA8 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDCA8 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse brain using CDCA8 antibody.
Immunohistochemistry of paraffin-embedded rat kidney using CDCA8 antibody.
Immunohistochemistry of paraffin-embedded human esophagus using CDCA8 antibody.
Immunohistochemistry of paraffin-embedded human stomach using CDCA8 antibody.
Borealin is a 280-amino acid protein that stabilizes the bipolar mitotic spindle. Borealin is part of a complex called Aurora B kinase that has essential regulatory roles at centromeres and the central spindle in cells undergoing mitosis. This complex makes up the enzymatic core of the chromosomal passenger complex (CPC). It is possible that the Borealin subunit directs the CPC to centromeric DNA. Deletion of Borealin delays mitotic progression and causes kinetochore-spindle to mis-attach. Deletion also causes an increase in bipolar spindles associated with ectopic asters. These extra poles, which form after chromosomes acquire their bipolar orientation, seriously disrupt chromosome allotment during anaphase. It is true that inappropriate chromosomal segregation and cell division may cause auneuploidy leading to cancer, so an abnormal expression of Borealin may lead to gastric cancer.
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