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Reactivity | Human Mouse Rat |
Tested applications | WB ICC IP |
Recommended Dilution | WB 1:500 - 1:2000 ICC 1:50 - 1:100 IP 1:20 - 1:50 |
Calculated MW | 34kDa |
Observed MW | Refer to Figures |
Immunogen | A synthetic peptide of human CDK2 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | df |
Synonym | CDK2;Cell division protein kinase 2;p33(CDK2); cyclin dependent kinase 2; p33 protein kinase |
Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).
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