CDK4-Monoclonal Antibodies

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CDK4

Qty


Total
$220
Catalog #
A0016
Antibody Type
Monoclonal Antibody
Gene ID
1019
Swiss Prot
P11802
Size
Species
Mouse
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Calculated MW34kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human CDK4
Storage BufferStore at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SynonymCDK4;CMM3;MGC14458;PSK-J3 ;
Images
  • A0016: image 1

    Western blot analysis of extracts of HepG2 cell and HeLa cell line, using CDK4 antibody.

Background

Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle (4).

Protocol

N/A

MSDS
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