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Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
Calculated MW | 66kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human COL10A1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Western blot analysis of extracts of various cell lines, using COL10A1 antibody.
Immunohistochemistry of paraffin-embedded rat lung using COL10A1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using COL10A1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using COL10A1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using COL10A1 antibody at dilution of 1:100 (40x lens).
This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).
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