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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
Calculated MW | 89kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human CUL3 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | be |
Synonym | CUL-3; PHA2E |
Western blot analysis of extracts of PC12 cell line, using CUL3 antibody.
Immunohistochemistry of paraffin-embedded human prostate using CUL3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using CUL3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cell using CUL3 antibody. Blue: DAPI for nuclear staining.
CUL3 (Cullin-3) is a member of the cullin-based ubiquitin ligase family. By interacting with Hrt1 and BTB domain containing proteins, the complex functions as a CUL3-based E3 ligase to bring specific substrates to ubiquitinylation and degradation (1). The CUL3 complex has been shown to target many substrates involved in cell cycle progression (2), transcription (3), development and differentiation (4,5).
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