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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 58kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human DKC1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | j |
Synonym | DKC1; DKC; CBF5; DKCX; NAP57; NOLA4; XAP101; |
Western blot analysis of extracts of various cell lines, using DKC1 antibody.
Immunohistochemistry of paraffin-embedded Human colon using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Human esophageal using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Human gastric using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Human gastric cancer using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Human kidney using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded liver cancer using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Mouse brain using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Mouse pancreas using DKC1 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of U2OS cell using DKC1 antibody. Blue: DAPI for nuclear staining.
This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene. Mutations in this gene cause X-linked dyskeratosis congenita, a disease resulting in reticulate skin pigmentation, mucosal leukoplakia, nail dystrophy, and progressive bone marrow failure in most cases. Mutations in this gene also cause Hoyeraal-Hreidarsson syndrome, which is a more severe form of dyskeratosis congenita. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
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