EGLN1-Monoclonal Antibodies

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EGLN1

Qty


Total
$220
Catalog #
A1151
Antibody Type
Polyclonal Antibody
Gene ID
54583
Swiss Prot
Q9GZT9
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Calculated MW46kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human EGLN1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationf
SynonymEGLN1;C1orf12;DKFZp761F179;ECYT3;HIFPH2;HPH2;PHD2;SM-20;SM20;ZMYND6 ;
Images
  • A1151: image 1

    Western blot analysis of extracts of various cell lines, using EGLN1 antibody.

  • A1151: image 2

    Immunohistochemistry of paraffin-embedded human kidney using EGLN1 antibody at dilution of 1:200 (x400 lens)

  • A1151: image 3

    Immunofluorescence analysis of MCF-7 cell using EGLN1 antibody. Blue: DAPI for nuclear staining.

  • A1151: image 4

    Immunofluorescence analysis of MCF-7 cell using EGLN1 antibody. Blue: DAPI for nuclear staining.

Background

PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway (1,2). PHD1 is highly expressed in a wide array of tissues whereas PHD2 and PHD3 are expressed mainly in heart and skeletal muscle (1,3). The mRNA levels of PHD are upregulated by HIF through the hypoxia-response element under low oxygen conditions (4-7). These three enzymes also exhibit different peptide specificity target proteins, PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564 (2,8). In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin (3,9).

Protocol

N/A

MSDS
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