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+1-888.733.6849
+1-617.299.7367 (Int’l)
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| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC ICC IF FC |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 ICC 1:50 - 1:200 IF 1:50 - 1:100 FC 1:20 - 1:50 |
| Calculated MW | 44 kDa |
| Observed MW | Refer to figures |
| Immunogen | Recombinant protein of human EGLN2 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Synonym | EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1; |
Immunohistochemistry of paraffin-embedded mouse testis using EGLN2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using EGLN2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using EGLN2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse prostate using EGLN2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
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