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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 79kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human EHHADH |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | LBP; ECHD; LBFP; PBFE; FRTS3; L-PBE; |
Western blot analysis of extracts of various cells, using EHHADH antibody.
Immunohistochemistry of paraffin-embedded rat kidney using EHHADH antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using EHHADH antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cell using EHHADH antibody. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.
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