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+1-888.733.6849
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| Reactivity | Human Mouse |
| Tested applications | WB IHC IF IP FC |
| Recommended Dilution | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:20 - 1:50 IP 1:20 - 1:50 FC 1:20 - 1:50 |
| Calculated MW | 36kDa |
| Observed MW | Refer to Figures |
| Immunogen | Recombinant Protein of human EIF2S1 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | j |
| Synonym | EIF2S1;EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A ; |
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).
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