ERBB2-Monoclonal Antibodies

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ERBB2

Qty


Total
$220
Catalog #
A0306
Antibody Type
Polyclonal Antibody
Gene ID
2064
Swiss Prot
P04626
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman
Tested applicationsWB IHC IF FC
Recommended DilutionWB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:20 - 1:50 FC 1:20 - 1:50
Calculated MW138kDa
Observed MWRefer to Figures
ImmunogenA synthetic peptide of human ERBB2
Storage BufferStore at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationa
SynonymERBB2;CD340;HER-2;HER-2/neu;HER2;NEU;NGL;TKR1;
Images
  • A0306: image 1

    Western blot analysis of extracts of HeLa cell line, using ERBB2 antibody.

Background

The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).

Protocol

N/A

MSDS
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