|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:100|
|Observed MW||Refer to figures|
|Immunogen||Recombinant protein of human FECH|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Western blot analysis of extracts of various cells, using FECH antibody.
Immunohistochemistry of paraffin-embedded human gastric using FECH antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat heart using FECH antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse lung using FECH antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse stomach using FECH antibody at dilution of 1:100 (x40 lens).
The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
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