GAPDH-Monoclonal Antibodies

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GAPDH

Qty


Total
$135
Catalog #
AC001
Antibody Type
Polyclonal Antibody
Gene ID
2597
Swiss Prot
P04406
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:1000 - 1:10000 IHC 1:50 - 1:100 IF 1:20 - 1:50
Calculated MW36kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human GAPDH
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SynonymGAPDH;G3PD;GAPD;MGC88685 ;
Images
  • AC001: image 1

    Immunohistochemistry of paraffin-embeddedrat pancreas using GAPDH antibody at dilution 1:100(400x lens).

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transport, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PACR and also loading control in electrophoresis and Western blotting. This antibody is a rabbit polyclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocol

N/A

MSDS
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