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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
Calculated MW | 63kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human GPI |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | AMF; NLK; PGI; PHI; GNPI; SA36; SA-36; |
Western blot analysis of extracts of various cell lines, using GPI antibody.
Immunohistochemistry of paraffin-embedded rat brain using GPI antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using GPI antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using GPI antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using GPI antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U20S cell using GPI antibody. Blue: DAPI for nuclear staining.
This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.
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