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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
Calculated MW | 92kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human GPLD1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | PLD; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1; |
Western blot analysis of extracts of mouse blood cells, using GPLD1 antibody.
Immunohistochemistry of paraffin-embedded rat heart using GPLD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using GPLD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using GPLD1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cell using GPLD1 antibody. Blue: DAPI for nuclear staining.
Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.
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