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Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
Calculated MW | 48kDa |
Observed MW | Refer to Figures |
Immunogen | A synthetic peptide of human GSK3B |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | bdimpqt |
Synonym | GSK3B; GSK-3β; |
Western blot analysis of extracts of various cell lines, using GSK3B antibody.
Western blot analysis of extracts of various cell lines, using GSK3B antibody.
Immunohistochemistry of paraffin-embedded rat kidney using GSK3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GSK3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using GSK3B antibody at dilution of 1:100 (40x lens).
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
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