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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 52kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human GSS |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | GSS;GSHS;MGC14098 ; |
Western blot analysis of extracts of various cell lines, using GSS antibody.
Immunohistochemistry of paraffin-embedded mouse lung using GSS antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of HeLa cell using GSS antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cell using GSS antibody. Blue: DAPI for nuclear staining.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]
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