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Reactivity | Human Mouse Rat |
Tested applications | WB IHC ICC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 ICC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 15 kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human H3F3A |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | H3F3; H3.3A; |
Immunohistochemistry of paraffin-embedded mouse brain using H3F3A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using H3F3A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse colon using H3F3A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using H3F3A antibody at dilution of 1:100 (40x lens).
Immunofluorescence
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
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