|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF IP ChIP|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human HAT1|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Western blot analysis of extracts of various cell lines, using HAT1 antibody.
Immunohistochemistry of paraffin-embedded mouse cancer using HAT1 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HAT1 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U2OS cell using HAT1 antibody.
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.
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