HDAC2-Monoclonal Antibodies

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HDAC2

Qty


Total
$220
Catalog #
A2084
Antibody Type
Polyclonal Antibody
Gene ID
3066
Swiss Prot
Q92769
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Calculated MW55kDa
Observed MWRefer to Figures
ImmunogenA synthetic peptide of human HDAC2
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationfip
SynonymRPD3; YAF1;
Images
  • A2084: image 1

    Western blot analysis of extracts of various cell lines, using HDAC2 antibody.

  • A2084: image 2

    Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody at dilution of 1:100 (x40 lens).

  • A2084: image 3

    Immunohistochemistry of paraffin-embedded human lung cancer using HDAC2 antibody at dilution of 1:100 (x40 lens).

  • A2084: image 4

    Immunohistochemistry of paraffin-embedded human liver cancer using HDAC2 antibody at dilution of 1:100 (x40 lens).

  • A2084: image 5

    Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC2 antibody at dilution of 1:100 (x40 lens).

  • A2084: image 6

    Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC2 antibody at dilution of 1:100 (x40 lens).

  • A2084: image 7

    Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody at dilution of 1:100 (x40 lens).

  • A2084: image 8

    Immunohistochemistry of paraffin-embedded human gastric cancer using HDAC2 antibody at dilution of 1:100 (x40 lens).

  • A2084: image 9

    Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody at dilution of 1:100 (x40 lens).

  • A2084: image 10

    Immunofluorescence analysis of A549 cell using HDAC2 antibody. Blue: DAPI for nuclear staining.

Background

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression (8-10). Both proteins are found together in numerous complexes including the nucleosome remodeling and deacetylation complex (NuRD), MeCP1, and the mSin3A corepressor complex.

Protocol

N/A

MSDS
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