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Reactivity | Human Mouse |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 116kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human HIP1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | HIP-I; ILWEQ; |
Western blot analysis of extracts of various cell lines, using HIP1 antibody.
Immunohistochemistry of paraffin-embedded human liver injury using HIP1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using HIP1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse heart using HIP1 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U20S cell using HIP1 antibody. Blue: DAPI for nuclear staining.
The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants.
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