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+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC ICC IF FC |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 ICC 1:50 - 1:200 IF 1:50 - 1:100 FC 1:10 - 1:50 |
| Calculated MW | 25 kDa |
| Observed MW | Refer to figures |
| Immunogen | Recombinant protein of human HMGB1 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Synonym | HMG1; HMG3; HMG-1; SBP-1; |
Immunohistochemistry of paraffin-embedded mouse brain using HMGB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HMGB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HMGB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HMGB1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
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