|Tested applications||WB IHC|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:100|
|Observed MW||Refer to figures|
|Immunogen||Recombinant protein of human HRG|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||HPRG; HRGP; THPH11;|
Western blot analysis of extracts of mouse pancreas cells, using HRG antibody.
Immunohistochemistry of paraffin-embedded rat lung using HRG antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HRG antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using HRG antibody at dilution of 1:100 (40x lens).
This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.
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