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Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
Calculated MW | 19kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human IL10 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | b |
Synonym | IL10; CSIF; GVHDS; IL-10; IL10A; TGIF; Interleukin-10; Cytokine synthesis inhibitory factor; |
Western blot analysis of extracts of various cell lines, using IL10 antibody.
Immunohistochemistry of paraffin-embedded mouse heart using IL10 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat kidney using IL10 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human trachea using IL10 antibody at dilution of 1:100 (x40 lens).
IL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat-3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS-1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6).
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