LDHA-Methylated Antibodies

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LDHA

Qty


Total
$220
Catalog #
A1146
Antibody Type
Polyclonal Antibody
Gene ID
3939
Swiss Prot
P00338
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Calculated MW37kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human LDHA
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SynonymLDHA;LDH-M;LDH1;PIG19 ;
Images
  • A1146: image 1

    Western blot analysis of extracts of various cell lines, using LDHA antibody.

  • A1146: image 2

    Immunofluorescence analysis of A549 cell using LDHA antibody. Blue: DAPI for nuclear staining.

Background

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.

Protocol

N/A

MSDS
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