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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:100 - 1:200 |
Calculated MW | 48kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human MAPK8 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | bprs |
Synonym | MAPK8;JNK;JNK1;JNK1A2;JNK21B1/2;PRKM8;SAPK1 ; |
Western blot analysis of extracts of various cells, using MAPK8 antibody.
Immunohistochemistry of paraffin-embedded human gastric cancer using MAPK8 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human lung cancer using MAPK8 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon using MAPK8 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human prostate using MAPK8 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human stomach using MAPK8 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U2OS cell using MAPK8 antibody. Blue: DAPI for nuclear staining.
The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).
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