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Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Calculated MW | 97kDa |
Observed MW | Refer to Figures |
Immunogen | A synthetic peptide of human MCM4 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | CDC21; CDC54; hCdc21; P1-CDC21 |
Western blot analysis of extracts of various cell lines, using MCM4 antibody. (1: NIH/3T3 cell lysate 2: Hela cell lysate 3: K562 cell lysate )
Immunohistochemistry of paraffin-embedded Human brain tissue using MCM4 antibody.
Immunohistochemistry of paraffin-embedded human B cell lymphoma using MCM4 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using MCM4 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MCM4 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using MCM4 antibody at dilution of 1:200 (40x lens).
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
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