|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200|
|Observed MW||Refer to Figures|
|Immunogen||A synthetic peptide of human MDM2|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Western blot analysis of extracts of various cell lines, using MDM2 antibody.
Immunohistochemistry of paraffin-embedded rat kidney using MDM2 antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded human stomach using MDM2 antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded mouse brain using MDM2 antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded Human colon using MDM2 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of A549 cell using MDM2 antibody. Blue: DAPI for nuclear staining.
MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).
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