NCK1-Polyclonal Antibodies

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NCK1

Qty


Total
$220
Catalog #
A0999
Antibody Type
Polyclonal Antibody
Gene ID
4690
Swiss Prot
P16333
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Calculated MW43kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human NCK1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SynonymNCK1;MGC12668;NCK;NCKalpha ;
Images
  • A0999: image 1

    Western blot analysis of extracts of various cell lines, using NCK1 antibody.

  • A0999: image 2

    Immunofluorescence analysis of A549 cell using NCK1 antibody. Blue: DAPI for nuclear staining.

  • A0999: image 3

    Immunofluorescence analysis of A549 cell using NCK1 antibody. Blue: DAPI for nuclear staining.

Background

NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).

Protocol

N/A

MSDS
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